Mushy Mayhem

[terrancefartbubbler]

Yep and lots of iso

 

[terrancefartbubbler]

I did 2 more rice packs a few days ago and 2 millet bags, the millet bags have gone off after 4 days there is a solid piece of mycelium.

 

[Goonie Goat]

I did 2 more rice packs a few days ago and 2 millet bags, the millet bags have gone off after 4 days there is a solid piece of mycelium.

Are you going to mix it into some straw or coco after or just try fruiting off the rice bags?

 

[terrancefartbubbler]

I will try fruiting the rice packs in a SGFC on perlite. The millet bags I will mix into Coco in a mono tub.

 

[Goonie Goat]

 

[Goonie Goat]

This is supposed to be a GT bag but I'm not entirely sure if this is mycelium, it's way fluffier than the agar wedges I put in and there was a very good chance there was already contamination on the agar with the mycelium.

I've been trying to get clean transfers from my mycelium cultures, and it's really doing my head in as there's this Bacteria or Yeast not entirely sure but its spreading through all my new agar containers.
I think I'm upto the 4th transfer now and it's pissing me off.. basically I take a wedge of what appears to be clean mycelium and transfer under a still air box. Everything seems great for 3 to 4 days then this shit just blooms out of nowhere.
I'm thinking maybe I can take another clone and dip it in some diluted peroxide, hoping it's strong enough to kill this shit without killing the mycelium also...

The blotches above the mycelium chunk, that's the stuff I'm talking about

 

[terrancefartbubbler]

I inoculated a bag last week within 2 days there was white fluff I thought must be mould today it is covering 50% of the bag not sure if it's fluffy mycelium or what, the substrate feels solid like mycelium. I've been waiting for it to turn green and stinky

 

[Goonie Goat]

I inoculated a bag last week within 2 days there was white fluff I thought must be mould today it is covering 50% of the bag not sure if it's fluffy mycelium or what, the substrate feels solid like mycelium. I've been waiting for it to turn green and stink

I'm thinking this is mycelium but there's just little patches of like a powdery white that could be contam, I'm not really messing around breaking the bag up, if it is a bit of contam then I'm hoping the myc can grow around it and contain it from spreading

 

[Orb]

when I was looking into the lazy persons approach to cloning I saw online the Agar plates with antibacterial agents - would u add them to ur next mix?

 

[Goonie Goat]

when I was looking into the lazy persons approach to cloning I saw online the Agar plates with antibacterial agents - would u add them to ur next mix?

I might add some amoxicillin to the next batch, good idea. But I did add some vanilla extract to these ones and the one you got, it's supposed to be antibiotic to an extent but also is beneficial for the growth of some mycelium they reckon.. hope your clone is doing well

 

[Aye Shroomer]

So there’s two kinds of mycelium growth.
Tometose and rhizomorthic ( spelling could be wrong there)
Rhizo has those branching arms
Tometose is white and fluffy

That agar transfer @Goonie Goat looks good. Just keep it going.
But agar work is easy to contaminate especially when just starting out. SAB only contain so much. Pathogens are always going to grow if they have the slightest chance.

 

[Goonie Goat]

So there’s two kinds of mycelium growth.
Tometose and rhizomorthic ( spelling could be wrong there)
Rhizo has those branching arms
Tometose is white and fluffy

That agar transfer @Goonie Goat looks good. Just keep it going.
But agar work is easy to contaminate especially when just starting out. SAB only contain so much. Pathogens are always going to grow if they have the slightest chance.

So I shouldn't need to worry about that blotchy stuff? I'll get some more pics of other ones

 

[Aye Shroomer]

Blotches can be the mycelium bruising
But they can be contams too. Hard to tell until it grows out. But if one side of the myc doesn’t have any blotches just keep transferring. So agar transfers need many attempts
Good you have a cheaper agar plate set up

 

[Goonie Goat]

Okay these are from the older plates which I have taken clones from, see the blotchy goo stuff. It covers the agar before the mycelium gets a chance to go rhizomorphic

GT


Hillbilly


Pan Cyan

 

[Goonie Goat]

On a side note, I think I have almost perfected my agar recipe lol

 

[Aye Shroomer]

Yeah ok they are certainly contams
Do you wear a face mask while doing agar work? Just limiting any possible vector helps. And gloves. But I found the nitrile ones are the best. When I ran out I just didn’t bother with gloves and washed my hands thoroughly. I noticed more contams after that.

 

[FreoJJ]

Ordering a couple of beginners bags and Jack Frost and Ecuador spores this weekend going to have a go

 

[Goonie Goat]

Yeah ok they are certainly contams
Do you wear a face mask while doing agar work? Just limiting any possible vector helps. And gloves. But I found the nitrile ones are the best. When I ran out I just didn’t bother with gloves and washed my hands thoroughly. I noticed more contams after that.

Yeah I do and have been spraying everything with alcohol, burning the blade etc etc, spraying down arms and hands with alcohol. Some of the takeaway containers don't seal up that well though, but those are older containers so I guess the chances of contam are higher the longer they sit around. Did a bit of digging on other forums and they reckon to drop an agar wedge into peroxide and leave it for 15 mins, will try that since I have lots of contaminated containers

 

[terrancefartbubbler]

Yea I'm gonna let

I'm thinking this is mycelium but there's just little patches of like a powdery white that could be contam, I'm not really messing around breaking the bag up, if it is a bit of contam then I'm hoping the myc can grow around it and contain it from spreading

Yea I'm gonna let the myc do its thing maybe do a break and shake later in life maybe around 70%

 

[Goonie Goat]

Yeah ok they are certainly contams
Do you wear a face mask while doing agar work? Just limiting any possible vector helps. And gloves. But I found the nitrile ones are the best. When I ran out I just didn’t bother with gloves and washed my hands thoroughly. I noticed more contams after that.

Yeah I do and have been spraying everything with alcohol, burning the blade etc etc, spraying down arms and hands with alcohol. Some of the takeaway containers don't seal up that well though, but those are older containers so I guess the chances of contam are higher the longer they sit around. Did a bit of digging on other forums and they reckon to drop an agar wedge into peroxide and leave it for 15 mins, will try that since I have lots of contaminated cont

Ordering a couple of beginners bags and Jack Frost and Ecuador spores this weekend going to have a go

Good luck with the bags, you doing syringes or swabs?