Mycelium Liquid Cultures

[Goonie Goat]

Decided to toss the check valves,
fresh air can pass through, and carbon dioxide can gas off when needed.

 

[Goonie Goat]

Pressure cooked liquid culture peptone, light malt and glucose syrup for about 15-20 mins at 12 psi.

All jars held up well just a matter of cooling down and injecting clean cultures into the jars now

 

[Goonie Goat]

Injected approx 2cc of liquid culture into each jar.

Going by the syringes I think the Lizard King will be the fastest colonizing strain of the lot.



Lizard King - Mexican
Koh Samui Super Strain - Thai
PE6 - Penis Envy × Texas
Moby Dick - Albino A+ × Golden Teacher

The peptone made the liquid change to a caramel colour.

 

[Hudo]

Let me know if you get the KSSS to fruit and how you achieved it, ive done all the above but couldn't for the life of me get the KSSS to fruit

 

[Goonie Goat]

Let me know if you get the KSSS to fruit and how you achieved it, ive done all the above but couldn't for the life of me get the KSSS to fruit

Hmm I wonder why it wouldn't?
My first grow I did albino a+ and had the same thing happen. Filled a whole monotub full of healthy white mycelium but just never pinned, then green mould took over.

What was your medium?

 

[Hudo]

Hmm I wonder why it wouldn't?
My first grow I did albino a+ and had the same thing happen. Filled a whole monotub full of healthy white mycelium but just never pinned, then green mould took over.

What was your medium?

Same its like it took to long and the mold beat it to the line, I tried straight coco, then I tried 70 coco 30 vermiculite, then 70/ 30 composted cowshit then gave up

 

[Goonie Goat]

Same its like it took to long and the mold beat it to the line, I tried straight coco, then I tried 70 coco 30 vermiculite, then 70/ 30 composted cowshit then gave up

I'll give them all a go in 2-1-1-.5 Cow shit, Coco, Vermiculite, Millet.

Probably just go with bags

 

[Hudo]

I'll give them all a go in 2-1-1-.5 Cow shit, Coco, Vermiculite, Millet.

Probably just go with bags

Last couple bags I done i put my brown rice in the bottom and then coco on top, sealed them 3/4 across about 50mm down then sealed the top 3/4 across. Got some of that polyester wool stuffing shit k-pock or whatever its called, packed it in between the 2 seal lines, then sealed the top the rest of the way and into the steriliser. Then when I wanted to start fresh air exchange I cut the corner off at the other end of the 3/4 seal ( only the top seal not the bottom seal line) so then i had about 120mm of filter the air had to pass through to get into the bag, then used my electric air bed pump to suck the old air out then blow new air in. You just have to watch you dont blow the stuffing into the bag, I done 8 bags and got zero mold and full bags of shrooms, next slow strain i get ill try this way that will buy it extra time without mold taking over

 

[Goonie Goat]

Last couple bags I done i put my brown rice in the bottom and then coco on top, sealed them 3/4 across about 50mm down then sealed the top 3/4 across. Got some of that polyester wool stuffing shit k-pock or whatever its called, packed it in between the 2 seal lines, then sealed the top the rest of the way and into the steriliser. Then when I wanted to start fresh air exchange I cut the corner off at the other end of the 3/4 seal ( only the top seal not the bottom seal line) so then i had about 120mm of filter the air had to pass through to get into the bag, then used my electric air bed pump to suck the old air out then blow new air in. You just have to watch you dont blow the stuffing into the bag, I done 8 bags and got zero mold and full bags of shrooms, next slow strain i get ill try this way that will buy it extra time without mold taking over

Have you ever tried just dumping freshly cooked medium into bags? I hate how the bags get wrinkly from pressure cooking and it really fucks me over when I try sealing them off haha.

Was thinking of doing a little bit of millet on the bottom for a grain spawn point.
I'm still pretty new to mushie growing never really took it seriously, few oysters and other gourmet varieties but never had much luck with cubensis. Actually did better growing pan cyan blue meanies which are apparently the hardest to grow.

 

[Hudo]

No I've always just pressure cooked the lot, when I do all in one bags. I cook the rice for about 10 mins so you can just squash it with some pressure, put some on the bottom then coco on top then when inoculating i squirter the inside of the bag so it runs down under coco and onto the rice

 

[Goonie Goat]

Okay well it seems like all the jars of cultures are doing great, mycelium has multiplied very fast since the day of inoculation.

Bad pics, hard to capture mycelium but there's an obvious difference in the amounts since I first injected.

Notice others have basically the same setup with the syringe filters after doing some research.

A list of parts for anyone interested.

{productName}

Find {productName} at Bunnings. Visit your local store for the widest range of products.

www.bunnings.com.au

3 Preserving Jars - 550ml - Kmart

Shop 3 Preserving Jars - 550ml at Kmart. Enjoy Australia-wide delivery or free click & collect on selected items.

www.kmart.com.au

Grommet

Use to install airline on system. Drill size hole and install grommet of your size. Push airline through to create watertight seal.

happyhydroponics.com.au

5 SYRINGE FILTER 0.22um, 13mm DIAMETER PVDF MEMBRANE | eBay Australia

::.Generation-Preserving.:: • 100% Original • High Quality • Fast Shipping 5 SYRINGE FILTER 0.22um 13mm DIAMETER PVDF MEMBRANE 5 x Syringe Filter for Liquid Culture jars and many other uses .22um filter Material PTFE so can be autocalved 13mm Diameter

ebay.us

Pardon our interruption...

And some 3-4mm vinyl tubing, cut to about a cm to push onto the ends of the syringe filters, so they can be jammed into the lid grommet for a tight seal.

Use an 18 gauge needle for inoculating, and for collecting use a 16 to 14 gauge needle that's big enough to slurp up the mycelium.

Generic recipe for liquid culture online

• 1500 ml water
• 30ml of glucose syrup (corn syrup)
• 2.5 grams of peptone
• 3.7 grams of light malt extract

mix well on the stove, chuck it into the jars with lids tightened loosely.
Pressure cook for 15-20mins.

Tighten lids well when finished, wait to cool and shoot up

 

[Orb]

super tidy setup per usual Goonie, how long approx do u reckon the mycelium will last in the jars?

 

[Goonie Goat]

super tidy setup per usual Goonie, how long approx do u reckon the mycelium will last in the jars?

I think they'll last at least upto 2 years if stored in the fridge.

 

[Goonie Goat]

super tidy setup per usual Goonie, how long approx do u reckon the mycelium will last in the jars?

Just got them in a cardboard box underneath my tables in the 5×10 right now. I'll give it a few weeks and then chuck them in the fridge.

 

[Orb]

Just got them in a cardboard box underneath my tables in the 5×10 right now. I'll give it a few weeks and then chuck them in the fridge.

2 yrs is legend, im guessing the mycelium continues eating (slowly) while its in the fridge?

 

[Goonie Goat]

2 yrs is legend, im guessing the mycelium continues eating (slowly) while its in the fridge?

I think some kinds do continue growing in the cold, I'm no pro but I think they kind of just sit inactive in a kind of stasis, if you could call it that.

The professionals freeze their mycelium (cryogenics?) so I'm guessing it can last a lifetime probably

 

[Orb]

cryo sounds interesting

I forgot about some that was in an agar plate and it appeared to consume the blue gel bit by bit, turned the whole thing white before it then went pale brown a few weeks later. reckon it lasted maybe 6-7 months all up

 

[Goonie Goat]

cryo sounds interesting

I forgot about some that was in an agar plate and it appeared to consume the blue gel bit by bit, turned the whole thing white before it then went pale brown a few weeks later. reckon it lasted maybe 6-7 months all up

Yeah agar definitely doesn't last as long as liquid cultures im guessing.

I think agar is a bit of a waste of time but still needed if using spore swabs.
Cloning the best mycelium growth etc..

Luckily with the liquid cultures somebody probably already selected tissue from the best mushrooms to use in the liquid cultures already so it takes a lot of guesswork and frustration out of the equation